SUMO Mutants, Ub Derivatives, and Ub Mutants

August 2005

SUMO Mutants
SUMO Lysine (K) to Arginine (R) Point Mutants
Mutation of lysine 11 to arginine renders SUMO-2 and SUMO-3 unable to form poly-SUMO multimers.  The conserved lysine at position 11 is the point of attachment for the C-terminal glycine of the preceding SUMO protein.  The function of SUMO chains has not yet been fully elucidated, but SUMO modification has been implicated in nuclear transport, chromosome segregation, transcriptional regulation, apoptosis and protein stability.
	SUMO-1 K16R , human recombinant Cat.#: ULM-712 SUMO-2 K11R, human recombinant Cat.#: ULM-752 SUMO-3 K11R, human recombinant Cat.#: ULM-762   Available soon, purified SUMO chains! Please inquire.

Ubiquitin Derivatives
The chemistry of ubiquitin conjugation is confined to the conformationally flexible and accessible C-terminal                  Gly-75-Gly76 residues, and the seven internal lysine residues which are critical for the formation of poly-ubiquitin chains.  These derivatives are fully functional and allow for convenient immuno-detection and affinity purification of ubiquitinated conjugates.
HA-Ubiquitin, human recombinant	His6-Ubiquitin, human recombinant
Cat.#: U-110	Cat.#: U-530
myc-Ubiquitin, human recombinant	GST-Ubiquitin, human recombinant
Cat.#: U-115	Cat.#: U-540
FLAG-Ubiquitin, human recombinant
Cat.#: U-120

Ubiquitin Lysine (K) to Arginine (R) Point Mutants
Ubiquitin lysine point mutants are ideal for the study of mono- or poly-ubiquitin chain linkage requirements.  They can be used for the reduction in poly-ubiquitin length or conjugation rates.
Ubiquitin K6R, human recombinant	Ubiquitin K33R , human recombinant
Cat.#: UM-K6R	Cat.#: UM-K33R
Ubiquitin K11R, human recombinant	Ubiquitin (K48R, K63R), human recombinant
Cat.#: UM-K11R	Cat.#: UM-K4863R
Ubiquitin K27R, human recombinant
Cat.#: UM-K27R

Boston Biochem is the world's provider of Ubiquitin Pathway Related Products. All of our products are manufactured in-house with a high commitment to quality and affordability. It is our mission to provide our customers with the most innovative reagents for their research needs. We are committed to continually adding new products to our product range to keep current with the discoveries and requirements of the Ubiquitin Proteasome Pathway related researcher.